A survey of tephritid fruit fly species and their parasitoids was conducted in the western portion of Santa Catarina state, Brazil. A total of 9,197 fruits belonging to 46 plant species in 24 families was collected. The parasitoids recovered were Aganaspis pelleranoi (Brèthes), Lopheucoila anastrephae (Rhower) (Figitidae), Doryctobracon areolatus (Szèpligeti), Doryctobracon brasiliensis (Szèpligeti), Odontosema anastrephae Borgmeier, Opius bellus Gahan, Opius sp., Utetes (Bracanastrepha) anastrephae (Viereck) (Braconidae), and Trichopria anastrephae Lima (Diapriidae). Agasnaspis pelleranoi is the most frequent parasitoid species found in the west region of Santa Catarina. We recorded the first occurrence of L. anastrephae in Santa Catarina. Parasitism ranged from 1.2 to 46.9%.